Wollman and Jacob
incubated mixtures of hfr bacteria and
antibiotic-resistant F- strains with
several marker genes, and placed them in a blender at various times to interrupt
the conjugating process.
The cells are grown on
antibiotic media so that only recipient cells were recovered.
Genetic recombination showed a linear progression with time.
leu are always transferred and are used in the initial screen for recombinants.
After about 10 minutes, recombination of the genes started to be detected , in the order
This oriented transfer of genes can be used to construct a conjugation map.