A tRNA molecule contains many modified bases and contains a series of stems and loops, folded into a 3-dimensional conformation.

The 3' end is the amino acid binding site, while the anticodon loop recognizes a codon on the mRNA.

The anticodon (CGI) of this tRNAala molecule specifies the amino acid alanine, and can base pair with the triplets GCU, GCC, and GCA due to wobble.