Base excision repair in E. coli.
1. A base pair mismatch is recognized by DNA glycosylase, which removes the base from the sugar, creating an apurinic/apyrimidinic (AP) site.
  • AP endonuclease makes a cut in the phosphodiester backbone at the AP site.
  • The gap is filled by DNA polymerase I and DNA ligase
  • The error is corrected.