Cosmids are created by combining parts of the lambda chromosome with parts of plasmids. The cosmid pJB8 contains:
  • an origin of replication (ori) that allows it to replicate as a bacterial plasmid,
  • a cos gene for packing phage DNA into protein coats,
  • an ampicillin resistance gene (amp),
  • a region containing restriction sites for cloning (BamHI, EcoRI, ClaI, and HindIII). Cosmids can carry up to 50 kb of inserted DNA.