To test the hypothesis that this cyclic change is a response to natural selection, Dobzhansky and his group devised a laboratory experiment. They constructed large population cages from which samples of D. pseudoobscura could be removed periodically and studied. They began with a population that was 88 percent CH and 12 percent ST. The flies were maintained at 25°C and sampled over a 1-year period. As shown in Figure 26-6, the frequency of ST increased gradually until it was present at a level of 70 percent. At that point, an equilibrium between ST and CH was reached. When the same experiment was performed at 16°C, no change in inversion frequency occurred. The researchers concluded that the equilibrium reached at 25°C was in response to the elevated temperature, the only variable in the experiment. Figure 26-6. Increase in the ST arrangement of D. pseudoobscura in population cages under laboratory conditions. The results of the study indicate that a balance in the frequency of the two inversions and the gene arrangements they contain is superior