In a study of cichlid in
Lake Tanganyika
were examined for short interspersed elements to track their
evolutionary relationship.
Photograph of an agarose gel showing PCR fragments generated from
primers that flank the AFC family of SINES. Large fragments
containing this family are present in all members of the
Lamprologini tribe of cichlids (lanes 9-20). DNA from the species
in lane 2 has a similar, but shorter fragment, which may represent
another repetitive sequence. DNA from other species (lanes 3-8 and
21-24) produce short, non-SINE-containing fragments.
A Southern blot of the gel probed with DNA from the AFC family of
SINES. AFC is present in DNA from all species in the Lamprologini
tribe (lanes 9-20), but not in DNA from other species (lanes 2-8
and 21-24).
A second Southern blot of the gel probed with the genomic sequence
at which the AFC SINE inserts. All species examined (lanes 2-24)
contain the insertion sequence. The larger fragments in