A three-point mapping cross involving the brown midrib (bm), virescent seedling (v), and purple aleurone (pr) genes in maize.

Following a DCO in a three-point mapping cross, the allele in the middle position will be flanked by the other two alleles. So a pr–v–bm sequence will show pr+–v–bm+ after a DCO (least frequent) event. This is not observed.