Denatured, single-stranded DNA fragments can reassociate
into complementary double strands.
The single-stranded DNA concentration (C) can be plotted against a logarithmic scale of the product of
C0t (initial concentration of DNA single strands), and t (time).
The half-reaction time C0t1/2 increases as the fragment size
increases,
so this can be used to estimate genome
sizes.
Repetitive DNA sequences in some eukaryotic DNA allow them to have much shorter
C0t1/2.
