Chapter 10: DNA Structure and Analysis

DNA: secret of photo 51 (Nap=572.8092 FRA, Aur VHS=VCT 572.86 NOV)
Z-DNA is left-handed
restore 10.4 nucleotides per turn.
in situ = in place, undisturbed
Molymod/Tinkertoy DNA
Paper chromatography of coffee?
1=A	2=B	3=R	4=B	5=D
6=D	7=C	8=D	9=D	10=B
11=C	12=A	13=A	14=A	15=B
16=D	17=E	18=A	19=E	20=B




1=... did not		2=DNase			3=Reverse
4=T			5=5-methyl uracil???

6=Rosalind		7=... 30%		8=antiparallel
9=5'–3'? 		10=TCATGCGATC

11=FISH			12=Shorter		13=RNase?
14=Protein		15=C-3' and C-5'!!!

16=...inside of the 	17=slowly		18=longer
19=The retrovirus	20=T

21=... directions	22=5' CAGTCAAGCAT 3
See chap 9 CD
  1. Martha Chase: lab assistant for Hershey, 1952 paper. USC PhD 1964.
  2. Sulfur-containing amino acids: CYS, MET
  3. Isotope: diff atomic mass neutrons; unstable, decay: emit weak alpha (He-4), beta (electron), high energy gamma radiation:
    Geiger detects alpha & beta; scintillation for gamma; P32 & S35 is beta
  4. Transfection: infection by only the viral nucleic acid
  5. Interferon: proteins of immune system - replication of viruses
  6. Esters formed in condensation reaction between alcohol and acid
  7. Tubercle Bacillus: Tuberculosis
  8. Angstrom = 0.1 nm
  9. Rosalind Franklin 100 hours exposure, died 1958 age 37 ovarian cancer
    Marie Curie age 66 aplastic anemia, Nobel Physics & Chemistry
  10. Z-DNA is left-handed
  11. Ultracentrifuge 100k rpm