restriction_map2 restriction enzymes are Sal ONE and Hind THREEE
pET f1 origin of replication: produce single-stranded plasmid
cDNA Reverse transcriptase discussed in ch18/retrovirus.html
chain-termination dNTP=deoxy nucleoside triphosphate
pulsed-field-gel-electrophoresis discussed in ch10/electrophoresis.html
direct-injection integration followed by artificial insemination?
Ti-plasmid Agrobacterium tumifaciens tumors: crown galls; lambda cos packs phage DNA into protein coats.
plasmid-library (recombinant colonies should be white, not blue?)
To lyse bacteria: freeze/thaw, sonication w/ lysozyme, Triton X-100 detergent. French Press (tiny pores), grinding frozen cells w/ silica powder.
YAC yeast has 16 chromosomes. Posttranslational: chaperones help folding, carbohydrates YAC Wockhardt produces yeast-based insulin in India, 2003.
Restriction defense: methylate own DNA.
Oocyte , polar body.
heat-stable Taq polymerase
oligo-dT =
topic: transgenic organisms

1=	2=	3=	4=	5=
6=	7=	8=	9=	10=
11=	12=	13=	14=	15=
16=	17=	18=	19=	20=
















1=		2=		3=
4=		5=

6=			7=		8=
9=			10=

11=	12=	13=
14=	15=

16=	17=		18=
19=	20=

21=			22=		23=
24=			25=

26=	27= 	28=