Chapter 6
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Part 1: Genes, Chromosomes, and Heredity
Genetic Analysis and Mapping in Bacteria and Bacteriophages
Review
Bacterial growth can be divided into three phases:
phase,
phase (
growth), and
phase.
Cells grown in liquid medium can be quantified by the
serial
technique.
Bacteria can undergo
, in which genetic information from one bacterium is
transferred
to another,
resulting
in
in the
latter.
F
+
cells contain a
(F)
factor
that has genes for sex
formation and allows the host to donate
DNA
during
. Recipient cells are usually converted to F
+
.
An
(high-frequency recombination)
strain
has an
F
factor,
and can donate
DNA
in an
fashion
to an F
-
cell.
Sometimes, an F factor is
from the chromosome of an Hfr cell, producing an
cell.
Transfer of an F' to an F
-
cell
results
in a
.
such as
Factors
contain one or more genes and replicate independently of the bacterial chromosome.
plasmids confer antibiotic
resistance.
Bacteria can also undergo genetic recombination by
, in which pieces of extracellular
DNA
are taken up by a cell and
into the
chromosome.
(phages) such as T4 are viruses that
infect
a host bacterium and use the host enzymes to
reproduce.
The number of phages produced following the infection of bacteria can be
determined
by the
assay.
Sometimes a
phage integrates its DNA into the bacterial chromosome in a
process
called
, and the viral DNA (
) is replicated with the host DNA.
Bacteriophages can mediate genetic
in their bacterial hosts in a
process
called
.
In
transduction,
DNA instead of
DNA is packaged in a phage particle and transferred to a recipient
host.
mapping in bacteriophages can be done by examining phage
mutations
that affect plaque
.
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Nov 5, 2008
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